tgfβ1  (Elabscience Biotechnology)

Journal: Marine Drugs

Article Title: Orally Administered Rhamnan Sulfate from Monostroma nitidum Significantly Inhibits Melanoma Metastasis in Lungs and Aorta of Mice Implanted with B16 Cells

doi: 10.3390/md24040126

Figure Lengend Snippet: ( A ) Changes in the expression of melanoma cell metastasis-related factors in the M + W group mice. The expression levels of melanoma cell metastasis-related factors in the blood and tissues of M + W group mice implanted with B16 cells were compared to those in the control group. Factors that significantly increased are indicated by upward arrows, factors that remained unchanged are indicated by horizontal dotted lines, and factors that significantly decreased are indicated by downward arrows. The combined effects of changes in the expression of each factor are thought to promote melanoma cell metastasis. ( B ) Changes in the expression of melanoma cell metastasis-related factors in the M + RS group mice. The expression levels of melanoma cell metastasis-related factors in the blood and tissues of the M + RS group mice implanted with B16 cells were compared to those in the M + W group. Factors that significantly increased are indicated by upward arrows, factors that remained unchanged are indicated by horizontal dotted lines, and factors that significantly decreased are indicated by downward arrows. The combined effects of changes in the expression of each factor are thought to suppress melanoma cell metastasis. Abbreviations: PAR, protease-activated receptor; TGFβ1, transforming growth factor β1; IL-6, interleukin 6; TF, tissue factor; TNFα, tumor necrosis factor α; F4/80, macrophage marker F4/80; Ly6G, lymphocyte antigen 6 complex locus G6D; PAD4, peptidylarginine deiminase 4; cisH3, citrullinated Histone H3; TM, thrombomodulin; uPA, urokinase-type plasminogen activator; TAFI, thrombin-activatable fibrinolysis inhibitor; TAFIa, activated TAFI; MMP, matrix metalloproteinase; Ang-2, angiopoietin-2; bFGF, basic fibroblast growth factor; Robo4, Roundabout homolog 4; E-cadherin, epithelial cadherin; Snail-1, small family zinc finger 1; Wnt, wingless and int-1; Wnt3a, Wnt family member 3a; LRP, low-density lipoprotein receptor-related protein; Frizzled, Frizzled class receptor.

Article Snippet: The levels of PAR1, PAR2, TF, TNF-α, TGF-β1, Ang-2, bFGF, β-catenin, vimentin, fibronectin, and Snail-1 in tissues were determined using commercially available ELISA kits: PAR1 (MBS753326, MyBioSource, San Diego, CA, USA), PAR2 (MBS4501658; MyBioSource), TF (ab214091; Abcam), TNF-α (KE10002, Proteintech), TGFβ1 (E-EL-M0051, Elabscience, Houston, TX, USA), Ang-2 (MANG20, R&D Systems), bFGF (bs-0217R, Bioss Antibodies), β-catenin (ADI-900-135; Enzo Life Sciences, Executive Blvd Farmingdale, NY, USA), fibronectin (OKCD05702, Aviva Systems Biology, San Diego, CA, USA), vimentin (ELK3731, ELK Biotechnology, Denver, CO, USA), and Snail-1 (LS-F2317-1, LS Bio, Shirley, MA, USA).

Techniques: Expressing, Control, Marker

Journal: Frontiers in Immunology

Article Title: Development and validation of a potency assay matrix for optimized and consistent manufacture of clinical mesenchymal stem/stromal cells

doi: 10.3389/fimmu.2026.1725191

Figure Lengend Snippet: MSC-derived EVs drive Treg polarization. (A) Representative flow cytometry plots and quantification of FoxP3 and CD25 expression in CD4 + T cells treated with different numbers of EVs or without EVs (Ctrl). Data are means ± SD. n = 4 technical replicates using one PBMC donors. (B) IL-10 levels in the supernatant of T cells incubating with a range of EV numbers or without EVs. Data are means ± SD. n = 3 technical replicates using one PBMC donors. (C) Replication index ratio of FoxP3-positive vs. FoxP3-negative CD4 + T cells treated with different numbers of EVs or without EVs. Data are means ± SD. n = 3 technical replicates using one PBMC donors. (D) Representative imaging flow cytometry images of CD4 + and CD8 + T cells incubated with CBO-labeled EVs for 24hrs. (E) ELISA TGFβ1 levels on EVs (4 x 10 11 EVs used for ELISA) from MSC-CM derived from three different MSC donors. Data are means ± SD. n = 3 technical replicates using three independent EVs preparations per MSC donor. (F) Frequency of FoxP3 + cells among CD4 + T cells treated with 4 x 10 11 EVs for 3 days in combination with 5µM SB431542 or 1µM A83–01 or vehicle control (DMSO) or incubated without EVs (Ctrl). Data are means ± SD. n = 3 technical replicates using one PBMC donors. (G) Frequency of FoxP3 + cells among CD4 + T cells treated with or without 4 x 10 11 EVs for 3 days ± 5µg/mL anti-TGFβ1 antibody or isotype control. Data are means ± SD. n ≥ 3 technical replicates using ≥ 3 different PMBC donors. Statistical significance was determined using one-way ANOVA with Tukey’s HSD test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

Article Snippet: TGFβ1 concentration was evaluated using the TGFβ1 ELISA kit (R&D Systems, DB100C) following manufacturer’s instructions.

Techniques: Derivative Assay, Flow Cytometry, Expressing, Imaging, Incubation, Labeling, Enzyme-linked Immunosorbent Assay, Control